In this study effect of supplementation of oil industry by-products of as energy source on daily feed intake. egg weight. egg yield, fat percentage and fatty acid composition of egg yolk in laying hens. 96 Hisex Brown layers with 36 weeks old were used in this study. Hens were divided into four prougs. Groups were fed diets consisting in rates of % 2.5 sunflower crude oil (HY), surtlower soapstock (SS), acidulated sunflower soapstock (AY) and volatile matters (UM) during 56 days. Egg production percentage of groups were found as 75.22. 78.81,83.38 and 73.75 %. Amount of feed consumed four 1 kg egg was founds lowest (2.04 kg) in group fed diet consisting acidulated sunflower soapstock (AY) and highest (2.27 kg ) in group fed diet consisting SS. There were no effeet on the egg yield, feed intake and spesific gravity of the addition of oil industry by-products. Total saturated faty acid percentaces of egg yolks of groups were found as 23.11 %. 26.29 %, 25.92 %, and 27.88 % respectively and total unsaturated fatty acid percentage of egg yolks of groups were found as 76.89 %, 73.71 %. 67.56 % and 71.08 % respectively. The highest value of omega-3/omega-6 fatty acids rate in egg yolk oil obtained from soapstock groups. As a result it was concluded that suplementation of diets with by-products ot tat industry instead of crude oll has no negative effects on performance. Egg production cost in AY group was determined lower than the other groups in addition to high level of omega 3 faty acids which play important role in preventing heart vessel diseases.