2013, Cilt 29, Sayı 1, Sayfa(lar) 024-029
Usefulness of a microsatellite test panel for cattle parentage testing in Turkey
Ercan Kurar1,3, Zafer Bulut2, Mehmet Nizamlıoğlu2
1Selçuk Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Konya, Türkiye
2Selçuk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Konya, Türkiye
3Selçuk Üniversitesi İleri Teknoloji Araştırma ve Uygulama Merkezi (İLTEK), Konya, Türkiye
Keywords: Cattle, microsatellite, parentage testing
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Aim: The objective of this study was to test usefulness a microsatellite test panel for parentage analysis in widely reared cattle breeds in Turkey.

Materials and Methods: In this study, genomic DNAs were used from 148 cattle. A total of eleven bovine microsatellite loci were selected from a list suggested by ISAG and FAO MoDAD. Genomic DNA samples were amplified by polymerase chain reaction (PCR). Alleles were determined by fragment analysis after capillary electrophoresis. General population parameters including allel numbers (Na), observed (Ho) and expected heterozygosities (He), deviation from Hardy-Weinberg Equilibrium (HWE) and power of exclusion (PE) at each microsatellite locus were calculated.

Results: A total of 150 different alleles were determined ranging from 6 to 17 at each locus. The Ho and He were ranged from 0.435 to 0.884 and 0.632 to 0.887, respectively. Locus based PE-1 and PE-2 were varied as 0.239-0.630 and 0.419-0.774 and a total PE-2 value was calculated as 0.999.

Conclusions: It was concluded that the test panel seems to be applied in cattle identification efforts; however, there is need for increasing the number of the locus in the panel and development of efficient multiplex systems.