pISSN 1309-6958
eISSN 2146-1953
| 2014, Cilt 30, Sayı 2, Sayfa(lar) 085-088 |
| [ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ] |
| Differential diagnosis of Fasciola hepatica from different animal hosts by PCR |
| Osman Selcuk Aldemir1, Mederle Narcisa2 |
| 1Department of Parasitology, Faculty of Veterinary Medicine, Adnan Menderes University, Isıklı, Aydin, Turkey 2Universitatea de StiinNe Agricole si Medicina Veterinara a Banatului Timisoara, Facultatea de Medicina Veterinara, Timisoara, Romania |
| Keywords: Differentiational diagnosis, Fasciola hepatica, RAPD-PCR, sheep, goat |
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Aim: The objective of this study was evaluated differentiational
diagnosis of Fasciola hepatica from sheep and goat by
PCR.
Materials and Methods: F. hepatica were obtained from sheep and goat. These parasites were washed several times with pH:7.4 phosphate-buffered saline and phenol extraction/ ethanol precipitation method was employed in genomic DNA extraction. In order to optimization of the PCR conditions, five primers were selected to amplify the DNA of each F. hepatica. Results: Both sheep and goat were amplified products in different numbers and sizes by RAPD-PCR technique. The results show that different primers gave different bands (fragments) and allowed to identify genetic variations of F. hepatica. These bands were species-specific to F. hepatica from goat and sheep. Conclusions: The RAPD-PCR method can be useful for the differentiational diagnosis of F. hepatica of sheep and goat. |
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