2010, Cilt 26, Sayı 2, Sayfa(lar) 109-112 |
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Serodiagnosis of Brucella canis infection in dogs by a dipstick enzyme immunoassay |
Uckun Sait Ucan1, Zeki Aras1, Ahmet Semacan2 |
1Department of Microbiology, Faculty of Veterinary Medicine, Selcuk University, Campus, Konya, Turkey 2Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Campus, Konya, Turkey |
Keywords: B.canis, EIA, iELISA, dog |
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Aim: A rapid and reliable test is needed by the veterinary
practitioners although the canine brucellosis can be diagnosed
by bacteriological, molecular and serological methods.
By this study, developing a dipstick EIA for a rapid serodiagnosing
the infection was aimed.
Materials and Methods: The test was compared with Rapid Agglutination Test iELISA. Results: Sensitivity and specificity of the test were 82% and 95%, respectively when iELISA was evaluated as gold standard. Conclusion: Since the test has a good degree of specifity it is recommended as a screening test in the field. |
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