Aim: The purpose of this study is determined that anatomical and morphometrical characteristics of cisternal and canalicular part of the hypoglossal nerve in Holstein cattle. Material and Method: In this study, 24 heads of adult Holstein cattle which obtained from the slaughterhouse were used. Samples were fixated by using classical method and dissected in anatomy laboratory. Results: It was seen that hypoglossal nerve as 46,04+7,29 rootlets left from 10,36 +1,01 mm. in diameter hypoglossal line which placed 7,90 ± 0.54 mm in length from fissura mediana laterally onto level of the decussatio pyramidum in Holstein cattle. It was found out, at the end of cisternal course 9,23±1,50 in lenght, rootlets made up 7,90±1,75 bundles and these bundles penetrated into the dura mater via 2,29+0,68 points. Bundles which were surrounded by dura mater passed through 1,50+0,62 hypoglossal canals and then reached fossa condylaris ventralis. At this point these bundles were joined each others and composed of a nerve trunk. Conclusion: This study demonstrates comprehensively anatomical and morphometrical properties of cisternal and canalicular part of the hypoglossal nerve.