In this study it was aimed to make differential diagnose of metabolic bone diseases caused by malnutrition and wrong management practices in calves by evaluating biochemical and radiological signs. As animal material, one hundred calves were used in various breed, sex and age. The differences between calcium, inorganic phosphorus and vitamin A blood levels based on biochemical analyses which are obtained from animals with rickets, juvenile osteopenia and hypertrophic osteopathy were found statistically significant (p<0.05). Blood parameters were detected as 8.64; 9.09 and 12.52 mg/dl in calcium, 5.93; 7.97 and 8.78 mg/dl in inorganic phosphate and 16.49; 28.73 and 31.61 mcg/dl in vitamin A respectively. There were no statistically significant differences amongst disease groups with respects to ALP activities and pH levels (p>0.05). It was determined that; rickets, juvenile osteopenia and hypertrophic osteopathy have similar radiological findings and by the way the importance of evaluating biochemical parameters beyond radiological findings especially in the differential diagnosis was concluded.