pISSN 1309-6958
eISSN 2146-1953
| 2012, Cilt 28, Sayı 1, Sayfa(lar) 015-020 |
| [ Türkçe Özet ] [ PDF ] [ Benzer Makaleler ] |
| Expression of Toll-like receptors (TLRs) in the equine peripheral blood mononuclear cells (PBMCs) during the early pregnancy and estrous cycle |
| Ercan Kurar1, Mehmet Osman Atli2, Aydin Guzeloglu1, Seyit Ali Kayis3, Ahmet Semacan4 |
| 1Department of Genetics, Selcuk University, Konya 2Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Dicle University, Diyarbakir, Turkey 3Department of Animal Science, Faculty of Agriculture, Selcuk University, Konya 4Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Selcuk University, Konya |
| Keywords: TLRs, PBMCs, equine, pregnancy |
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Aim: Practical, reliable and economical pregnancy diagnosis
method for as early as possible time of pregnancy in mares
is still requirement. Immune tolerance against allogenic fetus
is critically important during the early pregnancy. Tolllike
Receptors (TLRs) are known to be an important part of
innate immune system. Aim was to evaluate expression of
TLRs in peripheral blood mononuclear cells (PBMCs) during
the early pregnancy in mare.
Material and Methods: Blood samples were collected on days of ovulation (d0) and 8 during estrous and pregnancy from three same mares. Total RNA samples were isolated from PBMCs and cDNA synthesis was performed. Expressions of TLRs at mRNA levels were quantified by using realtime RT-PCR in duplicates. Relative Expression Software Tool (REST2009) was used for statistical analysis. Results: TLRs (1 to 10) were found to be expressed in PMBCs by RT-PCR. However, real-time PCR measurements failed to show effects of pregnant or cyclic status on expression of TLRs. Conclusion: According to these results, it may suggest that determination of TLRs gene expression in equine PBMCs during early pregnancy is not a good marker in understanding early equine pregnancy. |
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