The aim of this study is to report a tick infestation detected on the Mediterranean Chameleon (Chamaeleo chamaeleon) in the Demre district of Antalya Province, located on the southern coast of Türkiye. A tick specimen collected from the orbital area was morphologically identified as Hyalomma aegyptium (Linnaeus, 1758) using microscopic examination. Although Hy. aegyptium infestation is common in tortoises of the genus Testudo and some lizard species, it is infrequent in chameleons. This study represents a new host-parasite relationship for Hy. aegyptium in Türkiye, and in the world.