2004, Volume 20, Number 4, Page(s) 011-016 |
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Identification of Poultry Meat Using Random Amplified Polymorphic DNA (RAPD) Technique |
Ali Arslan, O. İrfan İlhak, Ö. Pelin Bozkurt, Pınar Şeker |
Fırat Üniversitesi Veteriner Fakültesi. Besin Hijyeni ve Teknolojisi Anabilim Dalı, ELAZIĞ |
Keywords: Poultry Meat, Identification, RAPD |
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In this study, meats of 8 poultry species (chicken, turkey, gull, ostrich, duck, goose, quail, partridge) ware identified by random amplified polymorphic DNA (RAPD) method using two different primers of 10 nucleotides each (primer 1- CAA TCG CCG T, primer 2- CCA CAG CAG T). A 45-cyclus PCR (each cycle; 45 s at 94°C, 45 s at 364C, and 60 s at 72°C) was performed for identification of poultry flesh. Results concluded that RAPD is a practical, rapid, and reliable method that can be used for identification of poultry meals in control of adulteration of consumers from exposure to the flesh thai the society normally does not consume.
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