Son Sayı: 2025 (Yıllık Cilt - Sürekli yayın modeli)
pISSN 1309-6958
eISSN 2146-1953
pISSN 1309-6958
eISSN 2146-1953
| 2005, Cilt 21, Sayı 1-2, Sayfa(lar) 065-069 |
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| Determination by RAPD-PCR of Relationship Degrees of S. aureus and S. intermedius Strains Isolated from Bovine Mastitis |
| Emine Arslan1, Leyla Açık2, U. Sait Uçan3 |
| 1Selçuk Üniversitesi Fen Edebiyat Fakültesi, Biyoloji Bölümü, KONYA 2Gazi Üniversitesi Fen Edebiyat Fakültesi, Biyoloji Bölümü, ANKARA 3Selçuk Üniversitesi Veteriner Fakültesi, Biyoloji Anabilim Dalı, KONYA |
| Keywords: RAPD-PCR, Staphylococ, mastitis, bovine |
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The aim of this study was to determine of relationship degree with RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) method of 23 of S.aureus and 7 of S.intemnedius strains isolated from bovine mastitis. Dendrogram obtained by RAPD results showed that all the isolates clustered in to four-main groups at a genetic similarity of less than 30% when analyzed with 10 random primers. While the highest relationship degree was 91.30%, the lowest relationship degree was found as 0.44%. This study shows that RAPD-PCR assayed with random primers can be successfully applied to assess the genetic relationship of S. aureus and S. intermedius strains isolated from bovine mastitis.
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