Gelecek Sayı: Haziran 2024
pISSN 1309-6958
eISSN 2146-1953
| 2013, Cilt 29, Sayı 2, Sayfa(lar) 087-091 |
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| Investigation of Canine Parainfluenza Virus Type 2 in dogs by immunofluorescence |
| Oğuzhan Avcı1, Oya Bulut1, Orhan Yapıcı1,2, Atilla Şimşek1, Sibel Hasırcıoğlu3, Sibel Yavru1, Mehmet Kale3, Irmak Dik1, Kamil Atlı1 |
| 1Selçuk Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Konya, Türkiye 2Kırgızistan-Türkiye Manas Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Bişkek, Kırgızistan 3Mehmet Akif Ersoy Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Burdur, Türkiye |
| Keywords: CPIV-2, dog, MDCK, immunofluorescence |
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Aim: It was aimed to determine the Canine Parainfluenza Virus
Type 2 (CPIV-2) in dogs by immunofluorescence (IF).
Materials and Methods: In this study nasal and conjunctival swab samples were obtained from 61 pet dogs with clinical signs of respiratory system. Swab samples (total 132) were passaged on Madin-Darby Canine Kidney (MDCK) cell cultures for three times. Cell culture supernatant obtained from last passage was tested for CPIV-2 by IF. Results: Swab samples were inoculated onto MDCK cell cultures and subjected to three passages and no CPE was observed. All cell culture supernatants were analyzed by IF but no positive results to be determined. Conclusion: Although all of the samples were determined as negative, CPIV-2 dogs showing clinical signs of respiratory system should be examined for the presence of both CPIV and other respiratoric viral agents. |
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