In ıhis study, level of genetic diversity was investigated in one pure (German Black Head) and two crossbreed (Hasmer and Hasak) sheep breeds. For this purpose, three microsatellite markers (OarFCB20, OarJMP29 and OarJMP5£) were used. It was observed thai genetic diversity between these breeds were high and Fst values were statistically significant (P<0.001). The Fst values calculated for Hasmer and Hasak were found statistically insignificant (P>0.05) and no genetic difference was observed between fhese two crossbreeds. However, F|$ values were lound statistically insignificant (P>0.05) and there was no departure from the Hardy Weinberg equilibrium in any of these populations. Kev Words: SheeD. Genetic Structure Microsatellite