2004, Cilt 20, Sayı 4, Sayfa(lar) 011-016
Identification of Poultry Meat Using Random Amplified Polymorphic DNA (RAPD) Technique
Ali Arslan, O. İrfan İlhak, Ö. Pelin Bozkurt, Pınar Şeker
Fırat Üniversitesi Veteriner Fakültesi. Besin Hijyeni ve Teknolojisi Anabilim Dalı, ELAZIĞ
Keywords: Poultry Meat, Identification, RAPD
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In this study, meats of 8 poultry species (chicken, turkey, gull, ostrich, duck, goose, quail, partridge) ware identified by random amplified polymorphic DNA (RAPD) method using two different primers of 10 nucleotides each (primer 1- CAA TCG CCG T, primer 2- CCA CAG CAG T). A 45-cyclus PCR (each cycle; 45 s at 94°C, 45 s at 364C, and 60 s at 72°C) was performed for identification of poultry flesh. Results concluded that RAPD is a practical, rapid, and reliable method that can be used for identification of poultry meals in control of adulteration of consumers from exposure to the flesh thai the society normally does not consume.